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Object To determine the content of muscone in preparation Methods Standard addition, DNP derivatization and HPLC with electrospray ionization tandem mass spectrometry (LC/ESI MS/MS) were utilized Results Identification of muscone hydrazone in the sample was based on the unique mass spectra of the standard Under an optimum condition peak corresponding to + ion and characteristic fragments for muscone hydrazone were observed ESI and APCI full scan m/z 419 MS/MS had the same relative molecular mass and fragmentation pattern The recovery was 102 5% with RSD ≤ 2 76% (n=5) Conclusion The qualitative and quantitative determination on muscone could be fulfilled simultaneously
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Objective: To study the content of alkaloids in Huanglianjiedu Decoction and its disassemble prescription. Methods: The contents of four alkaloids such as berberine, jatrorrhizine, coptisine, palmatine in Huanglian jiedu Decoction and its disassemble prescription were determined by HPLC. Results: The content of four alkaloids extracted by tranditional decoction had a tendency to low. On the coutrary, when Rhizoma Coptis and Cortex Phellodendri were decocted together the quantity of alkaloids was additive, and when two herbs above decocted with Radix Scutella and Fructus Gardeniae the quantity of alkaloids decreased obviously. Conclusion:This study can provide a basis for establishing rational quanlitative standard for Huanglianjiedu Decoction.
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Objective: To develop a HPLC method for simultaneous determination of catechins (EGC、DC、EGCG、EC、GCG、ECG) and caffeine in tea polyphenols and to quantify seven ingredients in twenty nine batches of tea polyphenols samples from thirteen producers. Methods: The study was achieved using a C 18 column with a methanol water 0.1% formic acid gradient elution system. Results: The peak resolutions of seven ingredients in mixed standards were all above 2; The resolutions in samples were all above 1.5 except EGCG; The average recoveries were 98.56%～100.14%. Conclusion: This method is accurate, stable, reproducible and suitable for quality control of tea polyphenols; the variations of contents are obvious among tea polyphenols samples from different even the same producers.
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Objective: To establish the analytical method for the characteristic electropherograms of PSP by High Performance Capillary Electrophoresis(Capillary Zone Electrophoresis). Method: Types, concentration and pH of electrolyte, voltage, temperature, length and size of column were investigated for the analysis of PSP and BSA which is as standard using capillary zone electrophoresis. Results: The optimum condition is that 10mmol?L -1 boric acid buffer, pH9.18, a column 50?m?57cm(HP, effective length of 48.5cm), 30KV, temperature 25 ?C ,DAD detection. The analysis is ended within 7min. Conclusion: The method is simple, rapid and reliable. The characteristic electropherograms of PSP can be established.
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An introduction of principle of chromatographic fingerprint spectrum and its application on guality standard of Chinese drug. The chromatogram is digitiged by its relative retention value. The digitized chromatogrzaphic fingerprint specturm of some chinese drug was obtained. This technic can be used to reseasch guality standard of chinese drug sucessfully.
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The amygdalin content in Runing Granules has been determined on the column RP-C_(15), using acetonitrile:water(1:6)as mobile phase and detection wavelength at 240nm.This method showed a high sensitivity,simple operation,good reproducibility and reliable result.It provide a certain base for the quality control of Chinesemateria medica and traditional Chi- nese patent medicine with amygdalin.
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AIM: To establish HPLC-digitized fingerprint spectrum (HPLC-DFPS) of Rhizoma Pinelliae so that it will be applied in quality control of Rhizoma Pinelliae. METHODS: A gradient separated method was applied. Column: Agilent Zorbax ODS C_ 18 ; Mobile phase: acetonitrile-water; Detection-wavelength: 210 nm; Flow rate: 0.5 mL/min; Column temperature: 25℃. RESULTS: Using above optimum chromatograph condition, the HPLC-DFPS and technic parameters of Rhizoma Pinelliae were established. CONCLUSION: Comparison of a group of characteristic peaks suggests that the HPLC-DFPS technology is feasible in quality control of Rhizoma Pinelliae.
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To review and summarize the studies on preparation of Rhizoma Pinelliae in the recent years, including cleaning processing, preparing technology, and influenc e on chemical ingredients and pharmacological effects caused by prepara tion.
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Objective: A HPCE capillary zone eletrophoretic method for the determination of evodiamine in Fructus Evodia was established. Similarly, areliable HPCE-FPS (fingerprint spectrum) was applied to compare quality of Fructus Evodia to 14 samples of 3 species from different areas. Methods: The evodiamine was detected on 254nm and proteins was on 196nm、254nm by HPCE. The electrophoretic parameters were optimized by studying the efffect of the buffer composition. Results: Calibration graph was constructed in the range 1.944 ?g?mL -1～7.766 ?g?mL -1 for evodiamine (r=0.9974). The regression equation was y= 188.7x-32.1. The average recovery was 101.3 (n=3). In this paper, the proteins of Frutus Evodia HPCE-FPS was established at 196nm and 254nm. Conclusion: Content of evodiamine from Fructus Evodia was determined by HPCE-CZE and HPCE-FPS and distinguished three species from different growing areas related to intrinsic quality of them. The research provides new technique for evaluating quality of traditional Chinese medicine.
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Objective: To establish the quality assessment of Cordyceps and its cultured Cmycelia by TLC fingerprint, and to compare the content of the nucleotides between them. Methods: Adenosine, Uridine and Guannosine were detemined by TLC-Scanning samples were perfomed by fluorescence quenching analyis. Results: The contents of adenosiue, uridine, and guanosine from cordyceps mycelia were more than that of cordyceps from wildlife. Conclusion: This method is convenient and rapid, and it can effectively evaluate nucleotides in nature Cordyceps and culture Cordyceps.
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AIM: To discuss the application of overlapping ratio to the fingerprints of plant and plant extract. METHODS: We took the HPLC fingerprints of green tea and tea polyphenols for decision procedure. RESULTS: Overlapping ratio could be applied to selecting representative samples for characteristic fingerprints of green tea and tea polyphenols,and could be used to deduce the raw material,process and producer of tea polyphenols.(CONCLUSION:) Overlapping ratio can be used to choose the suitable samples for establishing the characteristic fingerprint spectrum,and can supply references to relationship investigation among products,raw material and process.